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Bioinformatics analysis and qRT-PCR validation of iron metabolism-related genes in pediatric asthma

Fig 5

GO and KEGG enrichment analysis for IMRDEGs.

(A). Bubble diagram of gene ontology (GO) and pathway (KEGG) enrichment analysis of differentially expressed genes related to iron metabolism (IMRDEGs): biological pathway (KEGG), molecular function (MF), cellular component (CC), and biological process (BP). The abscissa shows GO and KEGG terms. (B–E). GO and pathway (KEGG) enrichment analysis outcomes of the iron metabolism-related differentially expressed gene (IMRDEGs) network diagram showing BP (B), CC (C), MF (D), and KEGG (E). Yellow nodes denote items, green nodes denote molecules, and the lines denote the relationships between items and molecules. The bubble size in the bubble plot indicates the number of genes, with the bubble color reflecting the p-value. A deeper red color denotes a smaller p-value, whereas a deeper blue color indicates a larger p-value. A p-value <0.05 and FDR value (q-value) <0.25 served as screening criteria for GO and pathway (KEGG) enrichment analysis. IMRDEGs: Iron metabolism-related differentially expressed genes, CC: Cellular component, KEGG: Kyoto Encyclopedia of Genes and Genomes, GO: Gene Ontology, BP: Biological process, MF: Molecular function, FDR: false discovery rate.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0346063.g005