Bioinformatics analysis and qRT-PCR validation of iron metabolism-related genes in pediatric asthma
Fig 2
Batch effect removal in combined GEO datasets.
(A). Box plots showing the distribution of the combined GEO datasets before batch removal. (B). Boxplots depicting the distribution of the same combined datasets after batch effect removal, illustrating the improved homogeneity across the datasets. (C). PCA plot showing the distribution of the datasets prior to batch effect correction. (D). PCA plot of the integrated combined GEO datasets following batch effect removal, demonstrating the improved clustering and reduced technical variation. In the PCA plots, the PA datasets are represented as follows: GSE27011 (orange), GSE40888 (green), and GSE40732 (purple). PCA: Principal component analysis, PA: Pediatric asthma, GEO: Gene Expression Omnibus.