Prostate cancer tissue mapping and stratification using DRAQ5 and Eosin fluorescent labels integrated with AI classification and segmentation algorithms
Fig 5
DRAQ5 images under different microscopy configurations.
These include a z-stack for different levels of focus (−5, −2.5, 0, 2.5, 5 um), confocal averaging for different levels of noise (X8, X4, X2, X1), multiple zooms for different levels of sampling density (pixel area size of 0.69x0.69, 0.52x0.52, 0.35x0.35 um2), and two different lenses (20X/0.8 M27, 10X/0.45 M27).