DNA source and primer choice affect the reliability of metabarcoding for nematode community profiling in agricultural soils
Fig 6
Relative abundance of nematode communities.
(A) Relative abundance of feeding groups across different DNA sources using the three primer sets, (B) Relative abundance of feeding groups in different agricultural soils, and (C) Relative abundance of nematode colonizer-persister (c-p) classification, pp is used to differentiate plant-parasitic nematodes from free-living nematodes. NE = Nematodes DNA extraction, SE = Soil DNA extraction, NemFopt = NemFopt–18Sr2bRopt, NemF = NemF–18Sr2b, and NF1 = NF1–18Sr2b.