DNA source and primer choice affect the reliability of metabarcoding for nematode community profiling in agricultural soils
Fig 5
Principal coordinates analysis (PCoA) of nematode community composition based on NF1, NemF, and NemFopt primers across soil types.
Points represent individual samples, colored by primer and shaped by soil type (clay vs. sand). Ellipses indicate 95% confidence intervals around primer-specific centroids. Axis percentages denote the proportion of variance explained by each principal coordinate.