DNA source and primer choice affect the reliability of metabarcoding for nematode community profiling in agricultural soils
Fig 4
Non-metric multidimensional scaling (NMDS) ordination plot illustrating differences among DNA sources plotted in species space across primer sets (NF1, NemF, and NemFopt).
The circles surrounding the clusters represent 90% confidence intervals. Each point represents a sample, and colors indicate extraction method. NE = Nematodes DNA extraction, SE = Soil DNA extraction, NemFopt = NemFopt–18Sr2bRopt, NemF = NemF–18Sr2b, and NF1 = NF1–18Sr2b. SE.10 = Soil DNA extraction using 10 g of dry soil, and SE.1.25 = Soil DNA extraction using 1.25 g of dry soil.