DNA source and primer choice affect the reliability of metabarcoding for nematode community profiling in agricultural soils
Fig 3
Hierarchical clustering heat map based on the relative abundance of the 20 most abundant nematode taxa.
Columns represent DNA extraction method and rows correspond to nematodes OTUs assigned at the genus and species levels. Dendrograms of hierarchical cluster analysis, grouping nematode genera and DNA extraction methods, are displayed on the left and top, respectively. Colors indicate standardized abundance values, with warmer colors representing higher relative abundance. NE = Nematodes DNA extraction, SE = Soil DNA extraction, NemFopt = NemFopt–18Sr2bRopt, NemF = NemF–18Sr2b, and NF1 = NF1–18Sr2b. SE.10 = Soil DNA extraction using 10 g of dry soil, and SE.1.25 = Soil DNA extraction using 1.25 g of dry soil.