In vitro culture of human-infecting Encephalitozoon spp. for genome sequencing with minimal host contaminant
Fig 5
Amplification of bacterial 16S, mammal and microsporidian rRNA in the E. intestinalis ATCC 50506 spore sample obtained from HFF-1 cell culture.
Ladder: 100 bp ladder (FroggaBio, ON, Canada). Neg: negative control, no sample. Well 1: E. intestinalis/E. hellem lsuF1-lsuR1 primers. Well 2: E. intestinalis/E. hellem lsuF2-lsuR2 primers. Well 3: E. intestinalis/E. hellem ssuF1-ssuR1 primers. Well 4: bacterial 357F-1100R primers. Well 5: mammal MF787- MR7535 primers. Bac + : Staphylococcus salivarius as positive control for bacteria primers.