Longitudinal assessment of fluorescence stability shows fluorescence intensity decreases over time: implications for fluorescence microscopy studies
Fig 6
Decrease in fluorescence intensity over time is dependent on secondary antibody fluorophore.
(a-c) Anti-GAP43 primary antibody and AF488-conjugated secondary antibody-stained OB section imaged with widefield microscopy. (d) Anti-GAP43-AF488 staining in GL shows a significant decrease in fluorescence intensity. (e) Anti-GAP43-AF488 staining in GCL shows a significant decrease in fluorescence intensity. (f-h) Anti-GAP43 primary antibody and AF546-conjugated secondary antibody-stained OB section imaged with widefield microscopy. (i) Anti-GAP43-AF546 staining in GL shows no significant change in fluorescence intensity. (j) Anti-GAP43-AF546 staining in GCL shows no significant change in fluorescence intensity. (k-m) Anti-GAP43 primary antibody and AF647-conjugated secondary antibody-stained OB section imaged with widefield microscopy. (n) Anti-GAP43-AF647 staining in GCL shows a significant decrease in fluorescence intensity. (o) Anti-GAP43-AF647 staining in GCL shows a significant decrease in fluorescence intensity. Images from weeks 1, 4 and 7 are shown as examples. Each line represents data from a single mouse.