Longitudinal assessment of fluorescence stability shows fluorescence intensity decreases over time: implications for fluorescence microscopy studies
Fig 5
OMP-AF488 stained OB sections showed a significant decrease in fluorescence intensity over time.
(a-c) Anti-OMP primary antibody and AF488-conjugated secondary antibody-stained OB section imaged with widefield microscopy. (d) OB sections imaged weekly show a significant decrease in fluorescence intensity. Images of weeks 1, 4 and 7 are shown as examples. Each line of data represents a single mouse.