Longitudinal assessment of fluorescence stability shows fluorescence intensity decreases over time: implications for fluorescence microscopy studies
Fig 3
Decreased fluorescence intensity of OE sections triple-stained for OMP-AF488, GAP43-AF546, and EdU-sulfo-Cyanine5 over the course of weekly widefield fluorescence microscopy.
Sections were triple-stained with anti-OMP primary/AF488-conjugated secondary, anti-GAP43 primary/AF546-conjugated secondary and EdU (click chemistry detection with sulfo-Cyanine5). (a-c) OMP-stained OE section. (d) Significant decrease in fluorescence intensity for OMP-stained OE over time. (e-g) GAP43-staining in the same OE section shown in a-c. (h) Significant decrease in fluorescence intensity for GAP43-stained OE over time. (i-k) EdU staining in the same OE section shown in a-c and e-g. (l) Trend towards a decrease in fluorescence for EdU-stained OE over time. Images of week 1, week 4, and week 7 are shown as examples. Each line of data represents an individual mouse.