Longitudinal assessment of fluorescence stability shows fluorescence intensity decreases over time: implications for fluorescence microscopy studies
Fig 2
Anti-OMP-AF488 and anti-HA-AF546 stained OE sections show significantly decreased fluorescence intensity over the course of weekly widefield imaging.
(a-c) Anti-OMP primary antibody with AF488 secondary antibody-stained section imaged with widefield microscopy. (d) Anti-OMP primary antibody staining combined with AF488-conjugated secondary antibody staining of OE sections show significant decreases in fluorescence intensity. (e-g) Anti-HA primary antibody-stained with AF546 secondary antibody-stained section imaged with widefield microscopy. (h) Anti-HA primary antibody staining combined with AF546-conjugated secondary antibody staining of OE sections show significant decreases in fluorescence intensity. Images of weeks 1, 4 and 7 are shown as examples. Each line of data represents a single mouse.