Tonsil-derived mesenchymal stem cells alleviate skin inflammation by modulating neutrophil extracellular trap formation and T cell migration
Fig 10
Transwell migration assay of Jurkat cells.
(A) Experimental design of the Transwell migration assay. Jurkat cells (human T cell leukemia line) were seeded in the upper chamber either alone, co-cultured with dHL-60 cells (neutrophil-like cells), or co-cultured with DNCB-treated dHL-60 cells. T-MSCs were placed in the lower chamber. Two pore sizes (5 µm and 8 µm) were tested. (B) Flow cytometry-based quantification of Jurkat migration under different conditions. Groups included Jurkat alone, Jurkat + dHL-60, Jurkat + DNCB-treated dHL-60, and each of these with or without T-MSCs. Migrated cells were quantified by flow cytometry for both pore sizes. (C) Quantification of Jurkat migration using 5 µm pore Transwells. Bar graphs show the percentage of migrated cells. (D) Quantification of Jurkat migration using 8 µm pore Transwells. Data were analyzed using two-way ANOVA, followed by Tukey’s post hoc test for multiple comparison (a: P < 0.001 for Jurkat vs. Jurkat + T-MSCs, b: P < 0.001 for dHL-60 + Jurkat vs. dHL-60 + Jurkat + T-MSCs, c: P < 0.001 for dHL-60 + DNCB + Jurkat vs. dHL-60 + DNCB + Jurkat + T-MSCs, d: P < 0.001 for Jurkat + T-MSCs vs. dHL-60 + Jurkat + T-MSCs, and e: P < 0.001 for Jurkat + T-MSCs vs. dHL-60 + DNCB + Jurkat + T-MSCs).