Tonsil-derived mesenchymal stem cells alleviate skin inflammation by modulating neutrophil extracellular trap formation and T cell migration
Fig 4
Histological analysis of inflammatory features by H&E staining.
(A) Representative H&E-stained dorsal skin sections from each group (Vehicle, Vehicle + T-MSCs, DNCB, and DNCB + T-MSCs) at 100 × magnification. Scale bar = 100 µm. (B) H&E-stained LN sections from each group at 4 × magnification. Scale bar = 50 µm. (C) H&E-stained spleen (SP) sections from each group at 4 × magnification. Scale bar = 50 µm. (D) Quantification of epidermal thickness across groups. Twenty measurements per group (5 per slide, 4 slides; n = 20) were included. (E) Quantification of LN medullary sinus area as a percentage of total LN area. Three slides per group (n = 3) were analyzed. (F) Quantification of splenic follicle area as a percentage of total SP area. Eight slides per group (n = 8) were analyzed. Images were analyzed with ImageJ, and results were analyzed by two-way ANOVA, followed by Tukey’s post hoc test for multiple comparison (* P < 0.05, ** P < 0.01, ***P < 0.001 and ****P < 0.0001). ns: not significant. Vehicle (V), vehicle + T-MSCs (V + T), DNCB (D), and DNCB + T-MSCs (D + T).