Tonsil-derived mesenchymal stem cells alleviate skin inflammation by modulating neutrophil extracellular trap formation and T cell migration
Fig 2
Neutrophil-like differentiation of HL-60 cells.
(A) Morphological changes in HL-60 cells during differentiation. Cells were treated with 0.8% dimethylformamide (DMF) and observed at Day 0 (undifferentiated) and Day 4 (differentiated). Images were captured using an Olympus DP28 microscope (original magnification, 200×). (B) Giemsa staining of differentiated HL-60 cells. Cells were collected by cytospin centrifugation, fixed in methanol, and stained with Giemsa solution. Images were captured using an Olympus DP28 microscope (original magnification, 200×). Differentiated HL-60 cells are indicated by arrows. Scale bar = 10 µm. (C) Flow cytometric analysis of neutrophil surface markers. Expression of CD11b, CD35, and CD71 was compared between Day 0 (undifferentiated) and Day 4 (differentiated) HL-60 cells.