Beyond the transcript: Chromatin implications in trans-splicing in Trypanosomatids
Fig 6
Differential protection at the TAS at single and multi-copy genes in Trypanosomes.
Schematic representation illustrating the proposed model of chromatin organization at the TAS in TriTryps. Consistent with lower levels of gene expression, multi-copy genes are encoded at genomic regions that are usually less accessible and their TAS are mostly covered by nucleosomes, while single-copy genes, that are transcribed more frequently, display a more open chromatin at the TAS. Moreover, TAS region at single-copy genes harbors R-loops, generated during the co-transcriptional maturation of transcripts, and some trans-splicing factors (TSF) interact through them.