Data-driven optimization of diet formulation to enhance survival and growth in Japanese Eel (Anguilla japonica) larvae
Fig 5
Comparison of survival rate and growth among diet groups in final validation trials (Tr_7-8).
(A) Survival rate and (B) mean TL from 6 to 100 dph, shown as line plots by diet group in each trial. Data points represent the mean values from replicate tanks (n = 2) for each diet at each sampling time point. (C) Box plots of TL at 100 dph for each diet group in Tr_7 and Tr_8. Boxes indicate the interquartile range, the horizontal line represents the median, and white diamonds indicate the mean. Different lowercase letters above the boxes denote significant differences among diets within each trial (Tukey-Kramer test, p < 0.05). Sample sizes (n) for each group are indicated above the x-axis. Note: In Tr_7 and Tr_8, the stocking density was adjusted at 41 dph. Therefore, after 41 dph, the survival rate was calculated by multiplying the cumulative survival rate up to 40 dph by the interval survival rate after 41 dph, using the number of larvae stocked at 41 dph as the new denominator.