Chronic intestinal immune activation reveals separable impacts of inflammation and barrier loss on hallmarks of ageing
Fig 5
PGRP-Lc overexpression suppresses cell junction gene expression and drives stem cell proliferation.
(A & B) Normalised mRNA level for Snakeskin (A, Ssk) and Mesh (B) in dissected whole gut samples from TiGS > UAS-PGRP-Lc non-Smurf and Smurf females fed RU486 and controls. n = 6 samples, 5 guts/sample. (C & D) Gut diameter at days 3 and 7 (C) and pH3 + cell counts at day 7 (D) post RU486 feeding from TiGS > UAS-PGRP-Lc non-Smurf females. (E) pH3 + cell counts at 25 days of age (15 days post RU486 feeding) from TiGS > UAS-PGRP-Lc non-Smurf and Smurf females fed RU486 and controls. N = (F & G) pH3 + cell counts in non-Smurf TiGS > UAS-PGRP-Lc female midguts at day 7 post RU486 and Gemcitabine feeding (F) and Smurf proportions at 15 days post RU486 and Gemcitabine feeding (23 days old) (G).Bar graphs show mean ± SEM. Boxplots display the 25–75th percentiles, with the horizontal bar at the median, and whiskers extending from the minimum to maximum points. Two-way Anova with Tukey’s multiple comparisons (A, B & E). Mann Whitney U (C & D) Kruskal-Wallis with Dunn’s post hoc test (F) Binomial test (G) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.