Chronic intestinal immune activation reveals separable impacts of inflammation and barrier loss on hallmarks of ageing
Fig 2
Overexpression of PGRP-Lc and intestinal barrier loss have distinct impacts on microbial growth.
(A-C) Internal bacterial levels assayed by qPCR of the 16S rRNA gene in surface sterilised females flies drug induced (RU50) from early adulthood and uninduced controls (RU0). n = 6 samples with 5 flies/sample. (A) 5966 > UAS-PGRP-Lc non-Smurf flies, (B) TIGS>UAS-PGRP-Lc non-Smurf and Smurf flies and (C) 5966 > UAS-PGRP-Lc flies monoassociated with Acetobacter pasteurianus (AP), Lactiplantibacillus plantarum (LP) or associated with a mix of both species. Boxplots show display the 25-75th percentiles, with the horizontal bar at the median, and whiskers extending from the minimum to maximum points. Two-way Anova with Tukey’s multiple comparisons. **p < 0.01, ***p < 0.001.