Parenterally administered pegbovigrastim alters leukocyte counts, granulocyte functions, and uterine cell population in healthy postpartum dairy cows
Fig 5
Effect of parenteral administration of pegbovigrastim on oxidative burst capacity of peripheral granulocytes.
(A) Representative flow cytometry plots show cell selection based on characteristic forward scatter (FSC) and side scatter (SSC) properties in combination with CH138A–allophycocyanin labeling to identify target cell population. (B) Comparison of granulocyte oxidative burst capacity between groups on different days post-treatment, expressed as the median fluorescent intensity (MFI) of rhodamine 123 signal. Postpartum cows were treated with pegbovigrastim (rG-CSF, recombinant granulocyte colony-stimulating factor, n = 12), or saline (Control, n = 11). Significant differences between treatment groups on specified sampling days are indicated by P < 0.05 in panel B.