Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids
Fig 5
Whole-mount immunofluorescence reveals protein expression throughout entire spheroids, preserving their 3D structure.
(A1-A8) Shown are single confocal images at different z-depths showing N-cadherin immunolabelling (red) and nuclei labelling with Hoechst stain (blue). Numbers in the upper left corner indicate the depth (in μm) of the optical sections within the Z-stack. (B) 3D reconstruction of a whole spheroid made from serial Z-stack images. The Z-stack comprised 274 optical sections, each taken with a 0.896 μm step. Scale bar, 200 μm.