Pulmonary fibroblasts activated by the addition of TNF-α and IL-4 enhance lymphangiogenic capacity and ameliorate lung fibrosis in an allogeneic rat model
Fig 5
Gene expression analysis and Lyve-1 localization in lung tissue.
(A) RT-qPCR analysis of fibrosis- and lymphangiogenesis-related genes in lung tissues. *P < 0.05, **P < 0.01 by one-way ANOVA followed by Tukey’s multiple comparison test. (B) Representative double-staining images combining WGA immunofluorescence (green) and Lyve1 ISH (red), with DAPI counterstain (blue). (C) Quantification of ISH signal (Total Intensity) in lung sections following rPF and rPF + CKs treatment.Total fluorescence intensity per image was measured in each group (Day 0, rPF, rPF + CKs, and Ctrl). Sample sizes were as follows: Day 0 = 9 images, Ctrl = 7 images, rPF = 7 images, rPF + CKs = 6 images. Welch’s t-test was performed to compare each group to the Ctrl. *p < 0.05 vs. Ctrl (Welch’s t-test).