Choosing the best route: Comparative optimization of wheat transformation methods for improving yield by targeting TaARE1-D with CRISPR/Cas9
Fig 5
Bar chart comparing survival and transformation efficiency across different methods.
a) represent hygromycin selection outcomes for immature embryo transformation depends on different parameters, b) for callus (from mature embryo) transformation, and c) show PCR screening results for in planta transformation. Statistical significance was determined at p = 0.01 (one-way ANOVA followed by Tukey’s HSD test). Bar charts were generated using three replicates of each optimization parameter. The raw data is available in S7 Table.