Choosing the best route: Comparative optimization of wheat transformation methods for improving yield by targeting TaARE1-D with CRISPR/Cas9
Fig 2
Transformation and regeneration of wheat plants from callus derived from mature embryos.
a) Callus was induced from mature embryos placed on culture medium. b) Subsequently used for transformation, the callus surface was covered with Agrobacterium suspension during transformation. c) Calli were transferred to selection medium (SM) supplemented with 0.2 mg/L IAA d) First shoots appeared within five days. e) Survived plantlets after four weeks of selection f) Surviving plantlets e were transferred to rooting medium. g) Well-developed plants in rooting medium. h) Acclimatized wheat in soil, they were maintained until seed harvest. Scale bar = 1 cm.