A remote surface loop modulates core structure and cold activity in phosphopantetheine adenylyltransferase
Fig 2
Multiple sequence alignment and phylogenetic analysis of MpaPPAT.
(A) Multiple sequence alignment of MpaPPAT with its homologues: Psychrophilic homologues, including MetPPAT (Methylocella tundrae) and MstPPAT (Methyloferula stellata); Mesophilic homologues, including EcoPPAT (Escherichia coli), MtuPPAT (Mycobacterium tuberculosis), and BsuPPAT (Bacillus subtilis); and Thermophilic homologues, including TthPPAT (Thermus thermophilus) and TmaPPAT (Thermotoga maritima). Highly conserved residues are highlighted in black, and partially conserved residues are shown in gray. The unique surface loop insertion (SCRLS in MpaPPAT) found in psychrophilic homologues is boxed in red. Positively and negatively charged residues lining the central funnel are indicated by blue and red diamonds, respectively. (B) Phylogenetic analysis of MpaPPAT homologues. The tree was constructed using the unrooted Neighbor-Joining method based on the alignment of the selected protein sequences.