Extracts from Cordyceps cicadae and Hericium erinaceus promote the neurite outgrowth of retinal ganglion cells
Fig 5
C. cicadae and H.erinaceus extracts at all concentrations do not affect cell apoptosis of retinal explants.
(A)-(G) Confocal images of P8 retinal explants cultured without and with C. cicadae and H. erinaceus extracts, respectively. Retinal explants were immunostained with cleaved caspase-3 (red) and nuclear marker DAPI (blue). (H) N6-(2-Hydroxyethyl) Adenosine (HEA; 100 μg/mL), C. cicadae-EtOH (Cc-EtOH; 100 μg/mL), C. cicadae-H2O (Cc-H2O; 100 μg/mL), Erinacine A (HeA; 10 μg/mL), Erinacine S (HeS; 5 μg/mL), and H. erinaceus-EtOH (He-EtOH; 100 μg/mL) did not significantly affect the apoptosis of retinal ganglion cells (n = 9 for the control; n = 7 for HEA; n = 8 for CC-EtOH; n = 9 for CC-H2O; n = 6 for HeA; n = 7 for HeS; n = 7 for HE-EtOH). Scale bar 100 μm. Error bars, mean ± SEM.