Extracts from Cordyceps cicadae and Hericium erinaceus promote the neurite outgrowth of retinal ganglion cells
Fig 2
Schematic diagrams of the quantification of the neurite outgrowth of retinal explants and retinal ganglion cells.
(A) The retinal explant images were split into two channels: blue (DAPI) and green (TUJ1). [1] The area of the retinal explant was defined using the blue channel, and the tissue perimeter was measured. [2] The total area of neurites expanded from the retinal explant was quantified. [3] The area outside the explant was divided into three regions: 100 μm (yellow), 100-200 μm (pink), > 200 μm (blue). Neurite areas grown out from the explants were measured in each of these regions. (B) The immunostained image of retinal ganglion cells consisted of blue (DAPI) and green (TUJ1) signals. [1] Neurite length was traced using Neuron J. [2] The length of grown neurite from the retinal ganglion cells was measured.