Anticonvulsant effects of novel and repurposed drugs on docetaxel-induced neuropathy in C. elegans
Fig 5
Acute treatment with sildenafil citrate decreases the duration of shock-induced seizure-like behaviors that accompany chronic exposure to docetaxel.
(A) Acute treatment with various concentrations of sildenafil citrate significantly decreases time to recovery for worms treated with chronic 0.005 mM docetaxel. Different letters denote a statistically significant difference in the mean values between the groups where “a” stands for not statistically significantly different from M9 saline, and “b” stands for statistically significantly different from M9 saline (Student-Newman Keuls, P < 0.05). Data shown as mean ± s.e.m. (B) Acute treatment with 0.10 and 0.25 mM sildenafil citrate decreases percent non-recovery following electroshock of nematodes exposed to chronic 0.005 mM docetaxel, while higher concentrations (0.5 and 1 mM) were less effective. 0.5 mM S.C. vs. M9, X2 = 0.0248, p = 0.8749; 0.005 mM DTX vs. M9, X2 = 5.1534, p = 0.0232; C.E. 0.005 mM DT vs.0.1 mM S.C. + C.E. 0.005 mM DTX, X2 = 5.3657, p = 0.0205; C.E. 0.005 mM DTX vs. 0.25 mM S.C. + C.E. 0.005 mM DTX, X2 = 9.6243, p = 0.0019; C.E. 0.005 mM DTX vs. 0.5 mM S.C. + C.E. 0.005 mM DTX, X2 = 1.6623, p = 0.1973; C.E. 0.005 mM DTX vs. 1 mM S.C. + C.E. 0.005 mM DTX, X2 = 2.756, p = 0.0969. N > 30 for each group. *, p < 0.05, compared to M9. #, p < 0.05; ###, p < 0.001; all compared to 0.005 mM C.E. DTX. S.C., Sildenafil Citrate.