A SNP-based capture and clustering workflow to assess donor-derived cell-free DNA in transplantation
Fig 2
Changes in alternative allele ratio in a mixing experiment using fragmentase-treated genomic DNA mimicking recipient cfDNA.
Fragmented genomic DNAs from two individuals were either left unmixed (A) or mixed at 1% (B) or 3% (C). These samples underwent capture hybridization and NGS sequencing to determine alternative allele ratios. In each plot, SNPs are sorted in descending order of alternative allele ratio. In panels (B) and (C), mixed samples are shown in orange and unmixed controls in dark blue. Reference alleles are denoted by A and a; alternative alleles by B and b. Uppercase letters indicate recipient-derived alleles, while lowercase letters indicate donor-derived alleles. The corresponding genotype combinations are illustrated in the figure.