Rapid detection of the beech leaf nematode, Litylenchus crenatae using recombinase polymerase amplification assay with lateral flow dipsticks
Fig 5
Lateral flow recombinase polymerase amplification (LF-RPA) assay with examples of lateral flow strips.
Sensitivity assay with crude nematode extract of Litylenchus crenatae (CD4200). Strips: 1: 1 nematode per tube reaction; 2: 0,5 nematode per tube; 3: 0.25 nematode per tube; 4: 0.125 nematode per tube; 5: 0.06 nematode per tube; 6: 0.015 nematode per tube; 7: 0.007 nematode per tube; 8: 0.002 nematode per tube; 9: negative control (no DNA).