Psmd13, a proteasome regulatory subunit identified in miR-29a regulation during neuronal differentiation
Fig 2
Screening of upstream candidate genes using neuronal differentiation in mNPCs.
(A-B) Role of candidate genes in neuronal differentiation. Representative images (A) and quantification (B) of βIII-tubulin positive cells using immunostaining in differentiated mNPCs transfected with individual siRNAs and scrambled siRNA negative control. N = 4 experiments. mean ± SD, **p < 0.01. One-way Anova. Image scale bars = 10 μm. (C-D) Representative histograms (C) and quantification (D) of βIII-tubulin detection using flow cytometry in differentiated mNPCs transfected with siPsmd13, siNap1l4 and siRNA of non-targeting control. N = 3 experiments. mean ± SD, *p < 0.05. One-way Anova. (E-F) miR-29a alter neuronal differentiation of mNPCs. Representative images (E) and quantification (F) of βIII-tubulin positive cells using immunostaining in differentiated mNPCs transfected with miR-29a inhibitor and miR negative control. N = 3 experiments. mean ± SD, *p < 0.05. Unpaired T-test. Image scale bars = 10 μm. (G-H) Representative histograms (G) and quantification (H) of βIII-tubulin detection using flow cytometry in differentiated mNPCs transfected with miR-29a inhibitor and inhibitor control. N = 3 experiments. mean ± SD, **p < 0.01. Unpaired T-test. (I-J) Psmd13 acts through miR-29a to alter neuronal differentiation. Representative images (I) and quantification (J) of βIII-tubulin positive cells using immunostaining in differentiated mNPCs transfected with siRNA non-targeting control, siPsmd13 plus mimic control, siPsmd13 plus miR-29a mimics and miR-29a mimics. N = 3 experiments. mean ± SD, **p < 0.01, *p < 0.05. One-way Anova. Image scale bars = 10 μm. (K-L) Representative histograms (K) and quantification (L) of βIII-tubulin detection using flow cytometry in differentiated mNPCs transfected with control plus siRNA non-targeting control, siPsmd13 plus mimic control siPsmd13 plus miR-29a mimics and miR-29a mimics. N = 3 experiments. mean ± SD, *p < 0.05. One-way Anova.