Enarodustat suppresses thymic stromal lymphopoietin expression via hypoxia-inducible factor-mediated c-Jun N-terminal kinases dephosphorylation
Fig 5
JNK inhibitor suppresses FSL-1-induced TSLP expression.
(A) Expression levels of phospho-JNK after SP600125 and FSL-1 treatment. Samples were collected 30 min after FSL-1 stimulation. Phospho-JNK and α-tubulin proteins were determined by western blotting. α-tubulin was used as a loading control. (B) Effects of SP600125 on FSL-1 induced TSLP mRNA expression. (C and D) Effects of T-5224 on TSLP mRNA expression levels induced by TNF-α (C) or PAR2-agonist + IL-4 (D). (E) The effects of SP600125 on TSLP mRNA expression induced by PAR2-agonist + IL-4. *p < 0.05, **p < 0.01 vs. stimulation alone group. Data are represented as the mean ± S.E.M. (n = 3-4). N.S.; not significant.