PS Poly: A chain tracing algorithm to determine persistence length and categorize complex polymers by shape
Fig 6
Process for determining overlapped polymers.
(A) Cartoon showing an example of how an overlapped polymer may form via twisting upon surface adsorption. (B) AFM image of CL polymer. The height is shown in greyscale, the lateral scale bar (yellow) is 20 nm. (C) Skeleton created from the data in panel B shows a potential branch point junction (arrow) comprising the intersection of 4 branches. (D) New skeleton that incorporates height information. (E) Skeleton created at a threshold of 1.5 times the average height of the polymer distal from the junction identifies the polymer as overlapped.