PS Poly: A chain tracing algorithm to determine persistence length and categorize complex polymers by shape
Fig 2
Polymer backbone isolation procedure.
The first steps to perform PS-Poly calculations on an AFM image of candidalysin are shown. Scale bars (yellow) are 100 nm. (A) Raw AFM image with topographic height, z, shown in greyscale. (B) Mask created from the AFM data. (C) New mask that has been expanded to a higher pixel density. (D) Skeleton created from the expanded mask. (E) Each molecule is registered as a unique object (pink circles).