Tissue-specific mitochondrial pathway remodeling linked to longevity in honeybee queens
Fig 5
Mitochondrial respiration pathway contributions in the abdominal fat tissue of worker and queen bees across ages.
Respiration is expressed as the flux control ratio (FCR), normalized to the maximal electron transport system (ETS) capacity after decoupling, as described in Fig 3. Panels show: (A) NADH-pathway contribution, (B) increase in flux following succinate addition (NS-pathway – NADH-pathway), (C) combined NS-pathway respication, (D) effect of glycerophosphate addition (NSGp – NS), (E) respiration after addition of rotenone (SGp-pathway), and (F) complex IV activity. Box plots display the minimum, 25th percentile, median, 75th percentile, and maximum values. Sample sizes; workers at 1 week (n = 8) and 4 weeks (n = 12); queen at 1 week (n = 6) and 109 weeks (n = 4). Two-way ANOVA p-values for caste, age, and their interaction are indicated. Significant differences within caste (below boxes) or between bee castes at a given age (above boxes) are denoted * (P ≤ 0.05), ** (P ≤ 0.01), and *** (P ≤ 0.001).