Molecular characterization and phylogenetic analysis of major envelope protein gene (B2L) and ATPase protein gene (A32L) of orf virus isolates from goats in Southern, Thailand
Fig 5
Scatter plots of ORF virus isolates based on discriminant analysis of principal components (DAPC) using A) B2L and B) A32L nucleotide sequences.
Individual viral isolates are represented as dots, and groups are indicated by inertia ellipses. The bar plot of discriminant analysis (DA) eigenvalues is shown in the inset panel. The first two discriminant functions correspond to the x- and y-axes, respectively. Genetic clusters are represented in different colors. A) B2L gene, Cluster 1 (light blue), Korea/2009 (GQ328006) and Brazil/1993 (JN088051); Cluster 2 (dark blue), China/2023 (PP733997) and Malaysia/2020 (OP279270); Cluster 3 (light green), Songkhla/2024 (PV173487, PV173488); Cluster 4 (dark green), Pattani/2024 (PV173489–PV173492), Malaysia/2018 (OK169620, MW537048), and Malaysia/2014 (KR024023, KR024024, KR024025); Cluster 5 (light pink), Pattani/2020 (PV173486) and Assam/2009 (JN846834); Cluster 6 (dark pink), Brazil/1992 (JN088052); and Cluster 7 (light orange), China/2024 (PP805860), China/2014 (KU199831), and China/2012 (KC568397). B) A32L gene, Cluster 1 (light blue), Ethiopia/2012 (KT438543, KT438544); Cluster 2 (dark blue), Ethiopia/2010 (KT438540) and Ethiopia/2008 (KT438539); Cluster 3 (light green), Pattani/2024 (PV173482–PV173485); Cluster 4 (dark green), China/2012 (KP010354, KP010356) and Assam/2010 (JN183069); Cluster 5 (light pink), New Zealand/1987 (DQ184476) and USA/1982 (AY386263); Cluster 6 (dark pink), Taiwan/2006 (EU327509); Cluster 7 (light orange), Ethiopia/2012 (KT438531–KT438533); and Cluster 8 (dark orange), Songkhla/2024 (PV173480, PV173481), Pattani/2020 (PV173479), Malaysia/2018 (MW537048), India/2018 (MH766377), India/2017 (MT332357), and USA/2000 (AY386264).