Characterization of a spontaneous microphthalmia-like mutant mouse with isolated ocular defects
Fig 7
H&E staining of eyeballs and of the mutant group and control group.
(A) Hematoxylin-eosin staining was performed on eye sections from the control and mutant groups at P28, P42, P56, P3M and P4M. A vertical section of the globe was taken through the optic disc plane. The red rectangle highlights areas that are depicted at higher magnification. Eyeballs in the mutant group exhibited the smaller size of the eyeballs and rosette-like structures in the retina compared to the control group. The retinal layer identifications: retinal pigmented epithelium (RPE), outer nuclear layer (ONL), inner nuclear layer (INL), and retinal ganglion cell (RGC). Representative histological data of the retina of mutant mice(n = 3) and normal mice(n = 3). Scale bar: 50µm (B) Measurement and statistics of the thickness of RPE of mutant mice(n = 3) and normal mice(n = 3) at P28, P42, P56, P3M and P4M. (C) Count and statistical analysis the retinal rosettes in the vertical section of mutant mice(n = 3) at P28, P42, P56, P3M and P4M.