A comparative investigation of catecholamines and glucocorticoids impact on glioblastoma invasive behavior via 2D and 3D cell culture
Fig 6
Microscopy and flow cytometry analysis of vimentin expression under different treatment conditions.
Rows 1–5 correspond to treatment groups:1: Epinephrine (2 μM), 2: Epinephrine (200 nM), 3: Control (untreated), 4: Hydrocortisone (500 nM), 5: Hydrocortisone (5 μM). Panels A1–A5: Flow cytometry density plots showing side scatter area (SSC-A) versus forward scatter area (FSC-A) for the ungated cell populations. Cell density is color-coded, with red indicating the highest and dark blue the lowest density. Panels B1–B5: Histograms of FITC fluorescence intensity in gated cell populations. Blue curves represent background fluorescence from unstained controls; orange curves represent cells stained with FITC-conjugated anti-vimentin antibody. Mean fluorescence intensity (MFI) values are shown for the stained populations. Panels C1–C5: Immunofluorescence microscopy images. (a) Fluorescent images: nuclei stained with DAPI (blue), vimentin with FITC (green); (b) Corresponding brightfield images. Scale bars = 100 μm.