LOTUS: A low-cost time-lapse automated imaging system for spatio-temporal analysis of microbial colony or biofilm development
Fig 5
Multimode imaging of biofilms with LOTUS.
Bright-field (epi-illumination and transillumination) and fluorescence images (green and red fluorescence channels) of biofilms containing one or two reporters were captured at biofilm growth experiment endpoint. Samples are: E. coli MG1655 promoter strains U66 (promoterless), U66+p5mCherry, rsd, and rsd+p5mCherry.