Fluid shear stress-dependent modulation of the basal endothelial glycocalyx
Fig 5
Comparison of apical and basal glycocalyx (GCX) expression in permeabilized human lung microvascular endothelial cells (HLMVECs) across fluid shear stress (FSS) magnitudes.
Sum projections (top image) and accompanying orthogonal view (bottom image) of the apical (A-C) and basal (D-F) GCX (green) and nuclei (blue) after 12 hours of exposure to 10 dynes/cm2 (physiologically normal)(B,E), 0.5 dynes/cm2 (pathophysiologically low)(A,D),and 30 dynes/cm2 (elevated)(C,F)FSS. Apical GCX thickness (µm), coverage (%), and integrated intensity (fold-change (F.C.) (G-I) are compared between FSS rates after 30 minutes and 12 hours of exposure. FSS comparisons are repeated for basal GCX thickness (µm), coverage (%), and integrated intensity (fold-change (F.C.) (J-L).