Effect of anticancer activity of aripiprazole main metabolite OPC-14857 on malignant glioblastoma
Fig 5
Effects of ARP or OPC on the formation and distribution of actin filaments.
A) Red shows F-actin stained by phalloidin-rhodamine conjugated and green shows G-actin stained by Alexa Fluor™ 488 DNase I. The right column shows the merged staining of F-actin and G-actin. Cells with membrane-localized F-actin are indicated by arrows. B) The fluorescence intensity ratio of ARP and OPC in F-actin and G-actin staining. The F-actin to G-actin (F/G) ratio was calculated using approximately 5 images. Statistical analyses were performed using Dunnett’s test. The Symbol *** (p < 0.001, n = 5) indicates significant differences between DMSO and ARP or OPC (30 µM).