Anatomical mapping and quantitative functional analysis of lower limb lymphatic flow in normal cynomolgus monkeys using indocyanine green lymphography
Fig 2
Examples of region-of-interest (ROI) selection and functional analysis of lymphatic contractility in a lower limb.
(A) Near-infrared fluorescence image of inguinal lymphatic vessels, with four ROIs indicated by pink squares. (B) Example of peak and valley assignment within an ROI (yellow squares), where valleys represent periods without ICG passage and peaks represent periods of ICG transit. (C) Peak–valley intensity plot and scalogram derived from the ROI shown in (B), generated using wavelet-based time–frequency analysis to illustrate contraction frequency and temporal variability.