Lysine p-nitroanilide impairs cellular energetics and potentiates statin-induced cytotoxicity in RD rhabdomyosarcoma cells
Fig 5
LPN is metabolized to PNA and alters WB stress markers.
T-test analysis of (a) aminoadipic acid and (b) myo-inositol levels in RD cells treated with 0.01 mM LPN for 48 hours. Stability analysis of LPN based on absorbance at 405 nm in RD cells after 72 hours of incubation (c). Viability assays using crystal violet staining in RD cells treated with LPN or PNA for 72 hours (d). Viability assays of RD cells pre-incubated with PNA for 24 hours followed by atorvastatin treatment (1/2 IC50) for 72 hours (e). Western blot analysis of RD cells treated with LPN or PNA [0.01mM] for 24 hours followed by atorvastatin treatment for 48 hours (f). Statistical differences between treatments and controls were assessed using one-way ANOVA followed by Dunnett’s post-hoc test. Significance levels: ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.