Fecal butyrate and deoxycholic acid quantitation for rapid assessment of the gut microbiome
Fig 5
Validation metrics for quantifying butyrate and DCA from fecal sample.
The analytical measurement ranges (AMR) and linearity of butyrate (A) and DCA (B) are shown across eleven concentration points for the rapid metabolomic screen. The lower limit of quantification (LLOQ) for butyrate at 3.71 µM (C) and DCA at 0.7 µM (D) are defined by the concentration at which the percent coefficient of variation (% CV) reaches 20%.