Human resistin is critical to activation of the NLRP3 inflammasome in macrophages
Fig 2
hResistin upregulates the expression of BTK, HMGB1, NLRP3, pro-IL-1β, pro-IL-18, and pro-caspase-1 and the cleavage of pro-IL-1β, pro-IL-18, and pro-caspase-1 in human macrophages.
Human THP-1–derived macrophages were cultured under the conditions shown for 24 hours. (A) Schematic diagram of proposed pathway. (B) Western blot images of HMGB1, NLRP3, BTK, pro- and cleaved caspase-1, pro- and cleaved IL-1β, and pro- and cleaved IL-18. (C) Quantitative analysis of each protein from panel B. Data represent means ± SD (n = 10 per group except the NLRP3 KO group, where n = 6). *p < 0.05, **p < 0.01, ****p < 0.0001. ab, antibody; Box-A, HMGB1 inhibitor; BTK, Bruton’s tyrosine kinase; Con, control; Ib, ibrutinib; HMGB1, high mobility group box 1; Hres, human resistin; IL, interleukin; KO, knockout; NLRP3, nucleotide-binding domain–like receptor protein 3; RAGE, receptor for advanced glycation end products. Created in BioRender. Lam, W. (2026) https://BioRender.com/1zhhut0.