Gene regulatory networks involved in activation of Notch signaling by AGEs in the pathogenesis of diabetic kidney disease
Fig 5
15 genes were found to be common upon cross-platform validation with RNA-Seq data.
A. Venn diagram representing 15 genes are common between signifiant RNA seq dataset and the 167 genes related to AGEs obtained from microarray dataset. B: Heatmap representing the logFC of expression values 15 genes from microarray dataset. C. Heatmap representing the logFC of the quantified transcript expression values 15 genes from RNA Seq dataset. DDIT3, GADD45A, THBS2, CCL2, and CSF1R showed consistent expression trends across platforms. D. Volcano plot showing the differentially expressed genes from microarray dataset GSE30122. 58 core genes from the condensed gene network along with the 5 genes that were consistently altered across microarray and RNA-seq dataset are highlighted. Genes significantly upregulated are shown in red, while significantly downregulated genes are shown in blue. E. Volcano plot showing the differentially expressed genes from RNA-seq dataset GSE299230. 15 genes from the condensed gene network from microarray dataset GSE30122 and RNA-seq dataset GSE299230 including the 5 genes that were consistently altered across microarray and RNA-seq dataset are highlighted. Genes significantly upregulated are shown in red, while significantly downregulated genes are shown in blue.