Identification of potential biomarkers and therapeutic targets for underactive bladder based on bioinformatics analysis and experimental validation
Fig 6
qPCR validation of DEGs and functional assays.
(A) qPCR validation of DEGs identified through CytoHubba and MCODE analysis in the PPI network. Among the six hub genes, except for C3 which showed no statistical difference, CLEC4E, CSF3R, CXCR2, and FPR2 were upregulated in the disease group compared to the control group, while IDO1 was downregulated. (B) Assessment of FPR2 knockdown efficiency by qPCR. (C) CCK-8 assay for assessing the viability of SV-HUC-1 cells. (D, E) Transwell assay to determine the migration ability of SV-HUC-1 cells (Scale bar, 100 μm). *: p < 0.05, **: p < 0.01, ***: p < 0.001. ns: no significance.