Photophysical image analysis for sCMOS cameras: Noise modelling and estimation of background parameters in fluorescence-microscopy images
Fig 5
Relationship of the estimated background Poisson parameter () with the exposure time of the sCMOS camera.
We show the average background Poisson parameter (over all tiles) for images with exposure times (1–1000 ms). The inset shows zoomed version of the image for 1–16 ms. Notice that increases linearly with exposure time, as it should (since the number of collected photons increases linearly with exposure time). The error bars are the standard deviations of
across tiles in the image.