Exploring β-catenin and TCF4 interaction in complex environments by means of novel biosensing platform focal molography
Fig 5
Overview of binding affinities (KD) and half maximum inhibitory concentrations (IC50) obtained in FM measurements performed in buffer.
The experiments were in triplicates on 3 different sensors. The boxplot analysis represents the indivudual binding affinities (A, KD) and half maximum inhibitory concentrations (B, IC50) calculated from 18 indivudual molograms with software provided by Lino Biotec. The box contains 50% of the values and the median value is represented as line, while the whiskers contain the upper and lower 25% of all molograms of an experiment. (C) Median KD and IC50 values calculated from 18 molograms as well as the avarage values calculated from three experiments with standart deviation.