Exploring β-catenin and TCF4 interaction in complex environments by means of novel biosensing platform focal molography
Fig 2
Backfilling the FM sensor surface with click chemistry.
Left: The time trace sensogram collected while backfilling of FM sensor with TCF4(7-30) peptide and its mutant. The median trace out of 18 molograms is shown. The coherent mass density increase is displayed on the y-axis. The x-axis shows the time dimension. Right: Scheme of backfilling process on the sensor surface. Initial surface chemistry; MTz in ridges and Tz in grooves (Step 1). In step 2, the non-binding mutant peptide was injected and primarily reacted with Tz in grooves. In step 3, since all Tz is depleted in grooves, the peptide only immobilized in ridges. In step 4, the binding peptide was only immobilized in the ridges until the signal decreased back to baseline. Step 5 depicts fully backfilled sensor surface. Figure was created with BioRender.